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運用仿生支架進行骨軟骨修復組織工程的生物設計策略

為了解決micron crucial的問題，作者Swathi Nedunchezian 這樣論述：

Acknowledgment iii摘要 vAbstract viiList of figures xiii1. Chapter One 1Introduction 11.1 Problem statement 11.1.1 Articular cartilage 31.1.2 Structure and composition of articular cartilage 31.1.3 Articular cartilage defect 51.2. Surgical techniques for cartilage and Osteochondral repair

currently in use 61.2.1 Bone marrow techniques 61.2.2 Mosaiplasty 81.2.3 Autologous chondrocyte implantation method 91.2.4 Matrix induced autologous chondrocyte implantation 111.3. Tissue engineering approaches to Osteochondral defect repair 121.3.1 Scaffold and hydrogel-based cell delivery 1

41.4. Cell source for tissue engineering purposes 161.4.1 Chondrocyte cells 161.4.2 Adult somatic stem cells 171.4.3 Bone marrow-derived stem cell (BMSCs) 181.4.4 Adipose-derived stem cells (ADSCs) 191.5 Scaffolds and hydrogels for tissue engineering 211.5.1 Natural hydrogels in cartilage tiss

ue engineering 251.6. Crosslinking of hydrogel for tissue engineering purpose 291.6.2 Silicon-dioxide Nanoparticle as crosslinkers in tissue engineering 341.6.3 Interaction of SiO2 nanoparticle with adipose-derived stem cells 361.7 Bio ceramics for Osteochondral tissue engineering and regenerati

on 371.7.1 Bio ceramics in Tissue engineering applications 371.7.2 Applications of bioceramics in Osteochondral tissue engineering 391.8 Research Objectives 421.8.1 The specific aims of this thesis are as follows: 43Chapter Two 44Characteristic and chondrogenic differentiation analysis of hybr

id hydrogels comprise of hyaluronic acid methacryloyl (HAMA), gelatin methacryloyl (GelMA), and the acrylate functionalized nano-silica crosslinker 442.1 Introduction 442.2 Materials and methods 522.2.1 Materials 522.2.2 Synthesis of HAMA hydrogel 522.2.4 Synthesis of acrylate functionalized nS

i crosslinker (AFnSi) 532.2.5 Identification of the synthesis HAMA and GelMA 542.2.6 Production of hybrid hydrogels 552.2.7 Identification of the synthesis AFnSi cross-linker 552.2.8 Fabrication of HG hybrid hydrogels 562.2.9.Swelling ratio evaluation 562.2.10 The microstructure morphology ana

lysis 572.2.11 Mechanical properties evaluation 572.2.12 In vitro degradation assay by hyaluronidase 582.2.13 Isolation and culturing of hADSCs 592.2.14 Cell viability assay 602.2.15 Chondrogenic marker gene expression 612.2.15 Quantification of DNA, sGAG deposition and collagen type Ⅱ synthes

is 622.2.16 Statistical analysis 632.3. Results and Discussion 632.3.1.Identification of the synthesis HAMA and GelMA 632.3.2 Identification of the AFnSi crosslinker 672.3.3 Swelling ratio of HG hybrid hydrogels 702.3.4 Morphological examination of HG hybrid hydrogels 722.3.5 Compressive stud

y of HG hybrid hydrogels 752.3.6.Viscoelastic property of HG hybrid hydrogel 782.3.7. Degradation study of HG hybrid hydrogels 812.3.8.Cell viability evaluation of hADSCs on HG hybrid hydrogels 822.3.8. Chondrogenic differentiation ability of HG hybrid hydrogels 852.4. Conclusion 90Chapter Thr

ee 92Multilayer-based scaffold for Osteochondral defect regeneration in the rabbit model 923.1 Introduction 923.2 Materials and methods 963.2.1 Preparation and Characterization of the 3D bioceramic scaffold by DLP method 963.2.2 Cell isolation and culture 973.2.3 Fabrication of the cell-laden

hydrogel/ 3D bioceramic scaffolds mimicking the Osteochondral tissue. 983.2.4 Surgery 983.2.5 Macroscopic Examination 993.2.6 Tissue Processing for paraffin block 993.2.7 Histological and Immunohistochemical Evaluation 1003.2.8 Masson’s trichrome stain 1013.3 Results and discussion 1023.3.1 C

haracterization of the 3D bioceramic scaffold by DLP method 1023.3.2 Fabrication of the hydrogel with hADSCs into the 3D bioceramic scaffold 1043.3.3 In-vivo studies using rabbit as an animal model 1053.3.5 Histological evaluation of neocartilage formation 1073.3.6 Masson’s trichrome staining an

alysis for neocartilage formation 1093.4. Conclusion 110Chapter four 1104.1 General discussion 1124.2 Future work 1134.2.1 Macroscopic Observation of neocartilage formation for 8 weeks 1145.Reference 115