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PPT template design進入發燒排行的影片

肽構像印記對於酶的活化

為了解決PPT template design的問題，作者KIRAN REDDY KANUBADDI 這樣論述：

The synthesis of selective catalytic materials is still a significant challenge. Peptide conformational imprinting technology (PCIT) offers a promising perspective to directly generate binding sites to preserve enzyme catalytic activity with high stability.In this study, first, we utilized peptide

conformational imprints (PCIs) to immobilize papain (PAP) at a targeted position, specifically on the surface of magnetic particles (MPs). Based on the flank part of the sequences on PAP, peptides were synthesized. As peptide fragment-mediated PCIs fabricated on magnetic particles (PCIMPs) with our

new cross-linker, three different templates were used to obtain PCIMPs with helical cavities that are complementary to the PAP structure. The best binding parameters of our PCIMPs 65-79 to PAP are Kd = 0.087 μM and Bmax = 4.56 μM. Upon esterification, N-Boc-His-OH was used to evaluate the activity o

f PAP/PCIMPs using proton nuclear magnetic resonance (1H-NMR). Finally, the kinetic analysis for esterification shows the best parameters of PAP/PCIMPs 65-79 had Vmax = 1.1×10-2 Mh-1, Km = 5×10-2 M, kcat = 1.1×10−1 s-1, and kcat/Km = 2 M-1 s-1. As PAP was bound tightly to the right site on PCIMPs, i

ts catalytic activities can sustain stable activity after four consecutive cycles.Secondly, the template chose based on the flank part of the porcine pancreatic alpha-trypsin (PPT). PCIMPs were prepared using the Metha-Asn-NHNH-Metha cross-linker for selective proteins PPT. We investigated protein a

ffinity towards using our synthesized PCIMPs via Metha-Asn-NHNH-Metha and also compared them with the traditional formulated PCIMPs using commercial cross-linker and monomers. Interestingly we found that fabricated using new cross-linker PCIMPs have shown high binding affinities towards the PPT with

the Kd = 0.20 µM and Bmax = 0.52 µM when compared with the traditional formulated PCIMPs. Moreover, the selective binding affinities were evaluated using the Bovine serum albumin (BSA), lipase, and PAP. We found that our PCIMPs have high binding towards their mother protein (PPT).